Collagenase I

Detailed Product Description

  

APPLICATIONS:

This product is suitable for the disaggregation of human tumor, mouse kidney, human adult and fetal brain,lung and many other tissues, particularly epithelium. It is also effective in liver and kidney perfusion studies,digestion of pancreas, isolation of nonparenchymal rat liver cells and hepatocyte preparation.

Although the term collagenase implies that there is a single enzyme produced by C. histolyticum, this is not the case. Crude collagenases are mixtures of enzyme (mostly proteases) secreted by C. histolyticum. All may contain 10 to 18 components (by electrophoresis), only 8 of which have been identified. The products differ by the amount of the components absolute and relative to each other.

The component enzymes in crude are two specific collagenases (measured as FALGA units/mg),

clostripain (measured as BAEE after reduction with DTT) and a neutral protease (measured as caseinase).

In the crude collagenase, the clostripain is mostly inactive, oxidized. An important feature for use in tissue dissociation is the ratio of collagenase to protease.

Effective release of cells from tissue depends on the action of both the two collagenase enzymes and the neutral protease, for either alone is not very effective.

STABILITY / STORAGE AS SUPPLIED:

This product is stable for at least one year when stored at -20°C. There is no loss in FALGPA or protease activity in 30 days at 37°C, 50°C and -20°C. 20