RACE Kit

Race Kitfor cloning the 3' end or 5' end region based on the sequence information of cDNA region already obtained

RACE Kit

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RNA can be analyzed by cloning and sequencing after target region was amplified through RT-PCR. However, in essence, there are many difficulties in obtaining full-length cDNA clones from mRNA. RACE (Rapid-Amplification of cDNA Ends) methods, which is performed for cloning the 3 end or 5 end region based on the sequence information of cDNA region already obtained, is effective to overcome these difficulties. 3 RACE Kit is a kit designed to specifically amplify the region which contains 3-end of target mRNA utilizing 3-RACE procedure. The supplied Oligo dT-3 sites Adaptor Primer is specially designed for efficient cDNA synthesis from 3-end of poly (A)+ RNA. 5' RACE Kit is a core set designed to perform the 5-RACE procedure. This set achieves amplification of an unknown 5-end region of mRNA utilizing inverse PCR and allows efficient 5-RACE. This set is optimized for the use with M-MLV Reverse Transcriptase. All the reagents required for cDNA synthesis from RNA and for the subsequent circularization of the obtained cDNA are supplied in this set.

Kit Component	3' RACE Kit	5' RACE Kit
M-MLV(200U/ul)	10µl	10µl
5×M-MLV Buffer	100µl	100µl
dNTPs(10mM)	40µl	40µl
RNasin(40U/ul)	10µl	10µl
Oligo-dT anchor Primer(20uM)	10µl	10µl
PCR anchor Primer	40µl	40µl
Taq Polymerase(5U/ul)	25µl	25µl
10×PCR Buffer	200µl	200µl
A tailing Enzyme(40U/ul)	-	10µl
dATP(20mM)	-	10µl
5×A tailing Buffer	-	100µl
RNase-free ddH2O	1ml	1ml